Tools for In Vitro Propagation/Synchronization of the Liverwort Marchantia polymorpha and Application of a Validated HPLC-ESI-MS-MS Method for Glutathione and Phytochelatin Analysis

Bryophytes, due to their poikilohydric nature and peculiar traits, are useful versatile organisms for studies on metal accumulation detoxification in plants. Among bryophytes, the liverwort Marchantia polymorpha is an excellent candidate as a model organism, having key role plant evolutionary history. In particular, M. axenic cultivation of gametophytes offers several advantages, such fast growth, easy propagation high efficiency crossing. Thus, main purpose this work was promote validate experimental procedures establishment standardized set-up gametophytes, well study cadmium processes terms thiol-peptide production, detection characterisation by HPLC-mass spectrometry. The results show how variations composition Murashige Skoog medium impact growth rate or development liverwort, what levels glutathione phytochelatins produced counteract stress.